Titlebook: RNAi and Small Regulatory RNAs in Stem Cells; Methods and Protocol Baohong Zhang Book 2017 Springer Science+Business Media, LLC, part of Sp

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RNAi and MicroRNA-Mediated Gene Regulation in Stem Cells. In this short review, we give a brief overview of the discovery history, functions, and mechanisms of RNAi and miRNAs. We also discuss the RNAi as a tool to study the stem cell function and the potential future practical applications.

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Forward RNAi Screens in Human Hematopoietic Stem Cellsogy. Here, we describe a method for RNA interference (RNAi)-based screens combined with next-generation sequencing (NGS) in primary human hematopoietic stem and progenitor cells (HSPCs). These cells are suitable targets for complex, selection-based screens using pooled lentiviral short hairpin RNA (

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Efficient Depletion of Essential Gene Products for Loss-of-Function Studies in Embryonic Stem Cellss essential for cell survival remains problematic, because genetic ablation of these genes results in cell death. As a consequence, cells recombined at the targeted gene and fully depleted of the gene product cannot be obtained. RNA interference is well suited for the study of essential genes, but t

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Regulation and/or Repression of Cholinergic Differentiation of Murine Embryonic Stem Cells Using RNAene. Regarding differentiation of cells into nervous system components, these techniques would play key roles in elucidating, not only the differentiation mechanisms of neuronal and glial cells but also how neuronal phenotypes are determined. In this chapter, we describe a RNA interference method fo

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A Recessive Genetic Screen for Components of the RNA Interference Pathway Performed in Mouse Embryonfy components of the mammalian RNAi pathway, we developed a recessive genetic screen in mouse embryonic stem (ES) cells. Recessive genetic screens are feasible in ES cells that are Bloom-syndrome protein deficient (Blm-deficient). We constructed a reporter cell line in Blm-deficient ES cells to isol