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Titlebook: RNAi and Small Regulatory RNAs in Stem Cells; Methods and Protocol Baohong Zhang Book 2017 Springer Science+Business Media, LLC, part of Sp

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發(fā)表于 2025-3-21 17:30:56 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱RNAi and Small Regulatory RNAs in Stem Cells
副標(biāo)題Methods and Protocol
編輯Baohong Zhang
視頻videohttp://file.papertrans.cn/821/820223/820223.mp4
概述Includes cutting-edge methods and protocols for the study of the intersection of RNAi, small regulatory RNAs, and stem cell biology.Provides step-by-step detail essential for reproducible results.Cont
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: RNAi and Small Regulatory RNAs in Stem Cells; Methods and Protocol Baohong Zhang Book 2017 Springer Science+Business Media, LLC, part of Sp
描述This detailed collection provides an accessible compendium of up-to-date methods focused on the study of RNAi and small regulatory miRNAs in stem cells. Beginning with a brief introductory section, the volume continues by exploring methods and protocols for RNAi screening, transfection, and the knockdown of specific genes and pathways in several animal species, including humans and mice, recently developed methods for miRNA expression and functional analysis, as well as usage of CRISPR/Cas 9 to knockout an individual gene for functional studies. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Practical and authoritative, .RNAi and Small Regulatory RNAs in Stem Cells: Methods and Protocols. aims to accelerate progress in this crucial field by reducing the time required to decipher and put into practice procedures published in the literature..
出版日期Book 2017
關(guān)鍵詞Stem cell differentiation; MicroRNAs; RNA interference; CRISPR-Cas9; Regenerative medicine; Gene knockdow
版次1
doihttps://doi.org/10.1007/978-1-4939-7108-4
isbn_softcover978-1-4939-8386-5
isbn_ebook978-1-4939-7108-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2017
The information of publication is updating

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發(fā)表于 2025-3-21 23:45:31 | 只看該作者
https://doi.org/10.1007/978-1-4939-7108-4Stem cell differentiation; MicroRNAs; RNA interference; CRISPR-Cas9; Regenerative medicine; Gene knockdow
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發(fā)表于 2025-3-22 02:56:35 | 只看該作者
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發(fā)表于 2025-3-22 08:35:41 | 只看該作者
RNAi and Small Regulatory RNAs in Stem Cells978-1-4939-7108-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
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發(fā)表于 2025-3-22 11:53:19 | 只看該作者
RNAi and MicroRNA-Mediated Gene Regulation in Stem Cells. In this short review, we give a brief overview of the discovery history, functions, and mechanisms of RNAi and miRNAs. We also discuss the RNAi as a tool to study the stem cell function and the potential future practical applications.
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發(fā)表于 2025-3-22 22:24:10 | 只看該作者
Gaoying Ren M.D.,Detlev Boisonor an der Berner Fachhochschule, Hochschule für Technik und Informatik, Biel. Der erste Band besch?ftigt sich mit dem elektrischen Energieversorgungsnetz. Besondere Bedeutung wird den modernen Methoden zur Modellierung der Dynamik der Netzelemente einger?umt.978-3-642-22346-4
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發(fā)表于 2025-3-23 02:40:19 | 只看該作者
1064-3745 ls.?.Practical and authoritative, .RNAi and Small Regulatory RNAs in Stem Cells: Methods and Protocols. aims to accelerate progress in this crucial field by reducing the time required to decipher and put into practice procedures published in the literature..978-1-4939-8386-5978-1-4939-7108-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
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發(fā)表于 2025-3-23 08:37:48 | 只看該作者
An Efficient Transfection Method for Differentiation and Cell Proliferation of Mouse Embryonic Stem ucing plasmids encoding the protein; (2) decreased SOX-2 and Oct-3/4 expression and subsequent morphological evidences of cell differentiation after introducing siRNA expression for suppressing SOX-2 and Oct-3/4, which are known to be essential for maintenance of stem cell properties in mouse ES cel
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