Titlebook: RNAi and Small Regulatory RNAs in Stem Cells; Methods and Protocol Baohong Zhang Book 2017 Springer Science+Business Media, LLC, part of Sp

寬大

RNAi Knockdown of Ape1 Gene in the Differentiation of Mouse Embryonic Stem Cellsentiation can be regarded as a valuable biological tool that has led to major advances in our understanding of cell and developmental biology. In vitro differentiation of mouse ES cells can be directed to a specific lineage formation, such as hematopoietic lineage, by appropriate cytokine and/or gro

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Implanting Glioblastoma Spheroids into Rat Brains and Monitoring Tumor Growth by MRI Volumetryve therapeutical approaches or to analyze the effect of protein overexpression or protein knockdown by RNA interference in vivo, animal models are mandatory. Here, we describe the implantation of C6 glioma spheroids into the rats’ brain and how to follow tumor growth by MRI scans. We show that C6 ce

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In vivo RNAi in the , Follicular Epithelium: Analysis of Stem Cell Maintenance, Proliferation, and D allows to control exactly where and when during development the function of a gene is depleted. The epithelium of the ovary is a particularly good model to study in a living animal how stem cells are maintained and how their descendants proliferate and differentiate. Here I provide basic informatio

恭維

A Phenotype-Based RNAi Screening for Ras-ERK/MAPK Signaling-Associated Stem Cell Regulators in ,tem cell homeostasis and normal animal development. Particularly, Ras-ERK/MAPK signaling pathway is critical for stem cell self-renewal and differentiation in mammals, including humans. Aberrant regulation of Ras-ERK/MAPK signaling pathway results in either stem cell or overproliferation. Therefore,

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Engineering Human Mesenchymal Stem Cells to Release Adenosine Using miRNA Technologyine-regulating enzyme, is critical to adapt its intra- and extracellular levels in response to environmental changes. Lentiviral RNAi-mediated downregulation of ADK in human mesenchymal stem cells (hMSCs) has therefore been considered an effective tool for engineering therapeutically effective adeno

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Book 2017s. Beginning with a brief introductory section, the volume continues by exploring methods and protocols for RNAi screening, transfection, and the knockdown of specific genes and pathways in several animal species, including humans and mice, recently developed methods for miRNA expression and functio

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