Titlebook: Melatonin; Methods and Protocol Ralf Jockers,Erika Cecon Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license

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Two-Dimensional Thin Layer Chromatography of Melatonin and Related Compoundsf culture media and homogenates of glands. The compounds resolved include N-acetylserotonin, melatonin, hydroxytryptophol, methoxytryptophol, hydroxyindole acetic acid, and methoxyindole acetic acid. The method requires either radiolabeled tryptophan or serotonin, if an investigator wants to study c

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Detection of Melatonin in Tissue Samplestter of organ and tissues’ sublocalization. It is of the highest importance to be able to precisely measure these quantities, and thus, these variations, particularly to better understand the way melatonin signals its presence and the variation thereof through many putative targets. In this chapter,

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Measuring Dim Light Melatonin Onset in HumansO) time is considered a gold-standard marker of the central circadian clock when measured from plasma or saliva. In this chapter, we describe the appropriate conditions for collecting plasma and salivary melatonin and the threshold method to calculate the DLMO.

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Measuring Urinary 6-Sulphatoxymelatonin in Humansy metabolite of melatonin, 6-sulphatoxymelatonin (aMT6s), is highly correlated with plasma melatonin and provides a noninvasive method to measure circadian phase, particularly in field-based studies. In this chapter, we describe the protocol for collecting urinary aMT6s and the method used to calcul

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Surgical Techniques and Nuances for Superior Cervical Ganglionectomy and Decentralization in Rats maintenance of the circadian rhythms, and neuronal disruption and recovery after injury. Of special interest is focus on the role of the superior cervical ganglion (SCG) in regulating the daily changes in pineal function. Removal of the superior cervical ganglion (SCGx) and decentralization have se

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Pineal Microdialysisneal gland throughout 24?h. It is a technique with great advantages that allows in vivo study of the ongoing pineal gland metabolism. In this chapter, we describe the entire process of pineal microdialysis that includes probe manufacturing, surgical procedure for its implantation, and the sample col