Titlebook: Melatonin; Methods and Protocol Ralf Jockers,Erika Cecon Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license

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Preparation of Single Pineal Cellsmetabolism, and cell-cell communication within the pineal gland. This procedure is the portal to single-cell RNA sequencing, which produces the transcriptome of individual cells. As such, single-cell RNA sequencing is critical to the continued development of knowledge of the pineal cell physiology.

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RNA Sequencing of Single Pineal Cellsing transduction processes and daily changes in gene expression and holds great promise in understanding development. Currently, the field is at an exciting point, with methods available for the isolation of individual cells and, as presented here, the preparation of these single cells for sequencin