Titlebook: Melatonin; Methods and Protocol Ralf Jockers,Erika Cecon Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license

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Analysis of the Human Pineal Proteome by Mass Spectrometryn day and night and/or between control and autistic pineal glands. This large-scale proteomic approach is the method of choice to study proteins in a biological system globally. This chapter proposes a protocol for large-scale analysis of the pineal gland proteome.

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1064-3745 tion advice from the experts.This volume covers the latest techniques that study the synthesis of melatonin, its receptor function, and its effects at the cellular and systemic level. The chapters are organized into three parts. Part One describes methods for the detection of melatonin and its biolo

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Radiochemical In Situ Hybridization in Developmental Studies of the Pineal GlandThe method combines determination of spatial and temporal gene expression profiles with semiquantitative analyses. We here describe the procedure of radiochemical in situ hybridization on the developing rat pineal gland ranging from preparation of fetal tissue for in situ hybridization to principles of quantification.

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Ralf Jockers,Erika CeconIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains keynotes and implementation advice from the experts

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