Titlebook: In Situ Hybridization Protocols; Boye Schnack Nielsen,Julia Jones Book 2020Latest edition Springer Science+Business Media, LLC, part of Sp

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Visualizing Genome Reorganization Using 3D DNA FISH in the reconstructed 3D images. In this way 3D DNA FISH provides a powerful tool to interrogate how the organization of specific genomic loci changes in response to stimuli. This chapter describes protocols which have allowed us to produce consistent data in cultured cells and paraffin-embedded tissue sections.

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Multiplexed Quantitative In Situ Hybridization with Subcellular or Single-Molecule Resolution Withinitation with subcellular resolution in an anatomical context and (2) dHCR imaging for digital mRNA absolute quantitation with single-molecule resolution in an anatomical context. Here, we provide protocols for qHCR and dHCR imaging in whole-mount zebrafish, chicken, and mouse embryos.

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Duplex In Situ Hybridization of Virus Nucleic Acids in Plant Tissues Using RNAscope?ruses in formalin-fixed paraffin-embedded tissue sections from cassava. The dual-color protocol described can be used as reference for virus/host interaction studies including the visualization of virus nucleic acids and plant endogenous mRNAs. RNAscope provides a specificity and sensitivity of target detection that otherwise cannot be reached.

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Book 2020Latest editionsearchers today. New developments in probe designs, detection systems, specificity and sensitivity improvements, and multiplexing combinations are explored. Chapters in this book are organized into seven sections and cover general applications; methods for DNA ISH; methods for cultured cells; method

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1064-3745 tion advice from the experts.This fifth edition volume expands on the previous editions with updated discussions on the many new in situ hybridization (ISH) techniques used by researchers today. New developments in probe designs, detection systems, specificity and sensitivity improvements, and multi

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Practical Application of Fluorescent In Situ Hybridization Techniques in Clinical Diagnostic Laboraty cancers. Multicolor FISH can be used to identify multiple targets, which can be simultaneously detected in individual cells using digital imaging microscopy. In an era of precision medicine there is a requirement to make a precise diagnosis and to have a molecular classification of the tumor that

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Fluorescent In Situ Hybridization Using Oligonucleotide-Based Probesbeen the most popular technique for chromosome identification in plants. Large insert genomic DNA clones, such as bacterial artificial chromosome (BAC) clones, and repetitive DNA sequences have been the most commonly used DNA probes for FISH. However, most of such traditional probes can only be used