Titlebook: In Situ Hybridization Protocols; Boye Schnack Nielsen,Julia Jones Book 2020Latest edition Springer Science+Business Media, LLC, part of Sp

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Visualizing Genome Reorganization Using 3D DNA FISHfluorescence in situ hybridization (3D DNA FISH) labeled probes complementary to specific loci of interest are hybridized to the genome. The samples are then imaged using fluorescence microscopy, collecting z-stacks through the nuclei, and the relative positions of the hybridized probes are analyzed

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Multiplexed Quantitative In Situ Hybridization for Mammalian or Bacterial Cells in Suspension: qHCR ial cells via flow cytometry. Third-generation in situ HCR (v3.0) provides automatic background suppression throughout the protocol, dramatically enhancing performance and ease of use. In situ HCR v3.0 supports analog mRNA relative quantitation via qHCR flow cytometry. Here, we provide protocols for

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cyanosis

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neoplasm

Duplex In Situ Hybridization of Virus Nucleic Acids in Plant Tissues Using RNAscope?y technology for probe design and hybridization assay. The method has been extensively used as a basis for sensitive diagnostic assays in the medical field, while applications of this technique in plant sciences are still rare. Here, we describe a multiplex ISH protocol for detection of two plant vi

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