Titlebook: In Situ Hybridization Protocols; Boye Schnack Nielsen,Julia Jones Book 2020Latest edition Springer Science+Business Media, LLC, part of Sp

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書目名稱In Situ Hybridization Protocols讀者反饋學(xué)科排名

溺愛

Boye Schnack Nielsen,Julia JonesIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains keynotes and implementation advice from the experts

細(xì)頸瓶

Methods in Molecular Biologyhttp://image.papertrans.cn/i/image/462991.jpg

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choleretic

RNAscope? Multiplex Detection in Zebrafishization studies. Presented here is a two-day protocol for robust multiplex detection of up to three different RNAs in zebrafish whole-mount embryos using the RNAscope procedure. Application of the protocol offers the simultaneous detection of multiple RNAs with a high signal-to-noise ratio in an intact embryo.

現(xiàn)實(shí)

Overcoming Autofluorescence (AF) and Tissue Variation in Image Analysis of In Situ Hybridizationue to masking of the specific signal across multiple wavelengths. Tissue sample variation can also affect levels of AF. In this chapter we share a method for overcoming the issues caused by sample variation and AF using HALO software on RNAscope in situ hybridization images.

COM

Guidelines for the Optimization and Validation of In Situ Hybridization, appropriate controls, and protocol optimization as well as a central core message of understanding the target, understanding the ISH technique, and using the most appropriate controlling mechanisms to enable reproducible and trustworthy data to be obtained.

模范

Multiplexed Detection and Analysis of Low-Abundance Long Noncoding RNA Using RNAscope? in Cultured Cplexed and robust method of detecting low-abundance RNAs, with improved signal-to-noise ratio using RNAscope-based RNA-FISH which utilizes a series of amplification steps. We have validated this protocol for investigating the regulation of low-abundance lncRNAs, which would be ideal for in vitro screening in 96-well plates.

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