Titlebook: Gene Therapy of Cancer; Methods and Protocol Wolfgang Walther Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), unde

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Climate Change and Arctic Securityenables localized transgene expression based on engineered vectors but also triggers immunogenic tumor cell death and elicits inflammation in the tumor microenvironment. Modified oncolytic viruses encoding immunomodulators have been developed to enhance antitumor immune effects and therapeutic effic

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R. Balasubramanian,V. Saravanakumaroncerns in clinical usage. Cell-based assays can detect RCA in batches of nonreplicating adenoviral vectors but cannot distinguish RCA from conditionally replicating oncolytic adenoviruses. Considering the great potential in using oncolytic viruses for cancer treatment, there is a need for comprehen

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Sahat M. Pasaribu,Abduh Sudiyantorventions, use of novel gene therapeutic technologies such as CRISPR/Cas, sleeping beauty vectors, and development of effective cancer immunogene therapies. In this chapter, a brief overview of current key developments in cancer gene therapy is provided to gain insights into the recent directions in

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https://doi.org/10.1007/978-3-319-92881-4ility requirement for a globally available therapy..One alternative method for engineering of T cells is the nonviral gene transfer by . (SB) transposition. Electroporation with two nucleic acids is sufficient to achieve stable CAR transfer into T cells. One of these vectors has to encode the gene o

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https://doi.org/10.1007/978-94-007-5757-8scribe assays that address transgene expression, biological potency and genomic vector copy numbers in polyclonal cell populations. Because transposons allow virus-free gene transfer with naked nucleic acids, the protocol can be adopted by any laboratory equipped with biological safety level S1 faci

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A Brief Introduction to Current Cancer Gene Therapy,rventions, use of novel gene therapeutic technologies such as CRISPR/Cas, sleeping beauty vectors, and development of effective cancer immunogene therapies. In this chapter, a brief overview of current key developments in cancer gene therapy is provided to gain insights into the recent directions in

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