Titlebook: Basic Cell Culture Protocols; Jeffrey W. Pollard,John M. Walker Book 19972nd edition Humana Press 1997

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Basic Cell Culture, It is not intended to describe the establishment of a tissue culture laboratory, nor to provide a historical or theoretical survey of cell culture. There are several books that adequately cover these areas, including the now somewhat dated, but still valuable volume by Paul (.), that of Freshney (.

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Establishment, Maintenance, and Cloning of Human Dermal Fibroblasts,Hayflick (.). He established a reliable protocol for the maintenance of fibroblast strains that was also favorable for stimulating cell proliferation. The reproducibility of the cell cultivation system he developed allowed the realization that normal diploid cells exhibit a limited proliferative pot

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Ex Vivo Maintenance of Differentiated Mammalian Cells,d dynamically interacting sets of mechanisms: Mechanisms associated with epithelial-mesenchymal interactions, a relationship that is universal and that constitutes the organizational basis for all metazoan tissues; and mechanisms associated with stem cell biology and with stem cell-fed maturational

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Scale-Up of Suspension and Anchorage-Dependent Animal Cells, that cells are now grown industrially in unit volumes of up to 10,000 L for vaccine, interferon, and monoclonal antibody production. The need to scale-up cell cultures has been expanded from the historical requirement for vaccine manufacture to include not only interferon and antibodies, but many i