Titlebook: Basic Cell Culture Protocols; Jeffrey W. Pollard,John M. Walker Book 19972nd edition Humana Press 1997

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Establishment, Maintenance, and Cloning of Human Dermal Fibroblasts,m a single primary culture derived from a biopsy. This bank of stable diploid cells thus has many attributes usually only found in transformed cell lines where homogeneity of cell population over a prolonged period of time is often crucial to the experimenter. Unlike a transformed line however, huma

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Scale-Up of Suspension and Anchorage-Dependent Animal Cells,complete expression and contamination with bacterial toxins, and more importance is now being put on expression of recombinant proteins from mammalian cells. This has allowed the use of faster growing and less fastidious cell lines, such as CHO, and amplification of product expression by multiple co

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Book 19972nd editionce and encouragement, in particular, Drs. L. Siminovitch and V. Ling. Elyse Rizzo‘s valuable secretarial assistance in compiling this new volume is greatly appreciated. Jeffrey Pollard John M. Walker W. Contents Preface ................................................................................

Myofibrils

,Sexualit?t im Alter — ein Tabu?,d the isolation of genetically pure strains. The cells described are all derived from Chinese hamsters since to date, these cells have proved to be the most useful for somatic cell genetics (.,.). The techniques, however, are generally applicable to most fibroblast cell types.

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Nachruf auf Professor Pierre Grappinrrow. This layer, termed “stromal” layer because of its likely derivation from the supportive elements in the marrow, has been extensively characterised (.). Stromal cells have been shown to exert positive and negative regulatory effects on hematopoietic cells through cell-cell interaction and by the secretion of cytokines (.,.).

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