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Titlebook: Basic Cell Culture Protocols; Jeffrey W. Pollard,John M. Walker Book 19972nd edition Humana Press 1997

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期刊全稱Basic Cell Culture Protocols
影響因子2023Jeffrey W. Pollard,John M. Walker
視頻videohttp://file.papertrans.cn/181/180967/180967.mp4
學(xué)科分類Methods in Molecular Biology
圖書封面Titlebook: Basic Cell Culture Protocols;  Jeffrey W. Pollard,John M. Walker Book 19972nd edition Humana Press 1997
影響因子It is now seven years since the original Animal Cell Culture volume was published. During this time, techniques have advanced dramatically. The- fore, we have completely revised this edition, including many new chapters and updating old chapters. There has also been a proliferation of methods books covering specific areas of tissue culture. Thus, in this revised edition, we have left out chapters on hybridoma technology because of the many books dealing specifically with this subject. Instead, we have concentrated on the original purpose of this volume now entitled Basic Cell Culture Protocols- which was to provide easy-to-follow protocols for animal cell culture, that hopefully, will be useful to experts and beginners alike. We would like again to acknowledge Dr. Cliff Stanners who taught me (JWP) cell culture, as well as members of the Toronto Somatic Cell Genetics Group for their advice and encouragement, in particular, Drs. L. Siminovitch and V. Ling. Elyse Rizzo‘s valuable secretarial assistance in compiling this new volume is greatly appreciated. Jeffrey Pollard John M. Walker W. Contents Preface ................................................................................
Pindex Book 19972nd edition
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Establishment, Maintenance, and Cloning of Human Dermal Fibroblasts,m a single primary culture derived from a biopsy. This bank of stable diploid cells thus has many attributes usually only found in transformed cell lines where homogeneity of cell population over a prolonged period of time is often crucial to the experimenter. Unlike a transformed line however, huma
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Scale-Up of Suspension and Anchorage-Dependent Animal Cells,complete expression and contamination with bacterial toxins, and more importance is now being put on expression of recombinant proteins from mammalian cells. This has allowed the use of faster growing and less fastidious cell lines, such as CHO, and amplification of product expression by multiple co
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Book 19972nd editionce and encouragement, in particular, Drs. L. Siminovitch and V. Ling. Elyse Rizzo‘s valuable secretarial assistance in compiling this new volume is greatly appreciated. Jeffrey Pollard John M. Walker W. Contents Preface ................................................................................
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,Sexualit?t im Alter — ein Tabu?,d the isolation of genetically pure strains. The cells described are all derived from Chinese hamsters since to date, these cells have proved to be the most useful for somatic cell genetics (.,.). The techniques, however, are generally applicable to most fibroblast cell types.
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Nachruf auf Professor Pierre Grappinrrow. This layer, termed “stromal” layer because of its likely derivation from the supportive elements in the marrow, has been extensively characterised (.). Stromal cells have been shown to exert positive and negative regulatory effects on hematopoietic cells through cell-cell interaction and by the secretion of cytokines (.,.).
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