Titlebook: Voltage and Patch Clamping with Microelectrodes; Thomas G. Smith,Harold Lecar,Peter W. Gage Book 1985 American Physiological Society 1985

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書目名稱Voltage and Patch Clamping with Microelectrodes影響因子(影響力)




書目名稱Voltage and Patch Clamping with Microelectrodes影響因子(影響力)學科排名




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Introduction,rical and chemical stimuli. In the last few years, extensions of the voltage clamp concept, (e.g., noise measurement) have been used to demonstrate how permeability change is mediated by the conformational transitions of discrete ionic channels, which constitute the fundamental molecular basis for electrophysiological events.

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Optimal Voltage Clamping With Single Microelectrode,Also, when using in vivo preparations, it is unlikely that the neurons can be visualized; penetrating such neurons with two independent electrodes or with a two-electrode combination is a difficult task.

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High-Resolution Patch-Clamp Techniques,rements of the amplitude and time course of currents arising from ensembles of many thousands of channels. With the patch clamp the transition rates between various conformational states of a channel and the current amplitude of each state can be measured directly for individual channels.

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Voltage Clamping Small Cells,small (<20 .m diam) cells. The whole-cell patch clamp, for example, takes advantage of the otherwise difficult properties of small cells and employs novel “tricks” to achieve the desired result of fast voltage clamping.

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Introduction,d chemically excitable cells. Voltage-clamp experiments lead to the, precise measurement of the membrane ionic currents underlying excitation. From these measurements it is possible to obtain exact descriptions of the ion conductances and transient conductance changes that occur in response to elect

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Microelectrode Shielding,tage-clamp and impedance measurements and for maintaining stability of the voltage clamp. Driven shields may also improve the accuracy of ion-selective electrodes by eliminating leakage currents across the walls of the pipette.

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Conventional Voltage Clamping With Two Intracellular Microelectrodes,ne potential in that area using microelectrodes to monitor potential and to pass current. This technique was first used successfully by Takeuchi and Takeuchi (23) to record currents generated by acetylcholine at the motor end plate.