Overview: The production of a book devoted to Vaccine Protocols has proved to be a complex task; the variety of procedures required to design, develop, pro- duce, and assess a vaccine is immense and covers aspects of chemistry, bio- chemistry, molecular biology, and immunology. Many of the previous volumes in the Methods in Molecular Biology series provide the background required for work on vaccines; of particular interest are the volumes on Immunochemi- cal Protocols, Practical Molecular Virology, Proteins, and Nucleic Acids. In the volume on Vaccine Protocols, we have tried to cover the lat
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