Titlebook: Stem Cell Nanotechnology; Methods and Protocol Kursad Turksen Book 2020Latest edition Springer Science+Business Media, LLC, part of Springe

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,Methodology Involved in the Osteogenic Differentiation of Mesenchymal Stem Cells on Chitosan–Collagof MSCs from bone marrow, seeding on fabricated nanofibers, and differentiation into osteoblasts is reported. The methods and techniques involved such as MTT assay, qRT-PCR, ALP activity, and immunofluorescence staining are also highlighted to investigate the potential of multifunctional nanofibers for the development of bony tissues.

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1064-3745 expertsThis fully updated edition brings together a new set of protocols to arm stem cell biologists with tools and approaches to continue the quest to uncover the intricacies and regulatory circuits underlying stem cell biology. The collection explores the tremendous progress in our understanding

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Design of a Versatile Sample Holder for Facile Culture of Cells on Electrospun Membranes or Thin Pomogeneous endothelium in vitro. Here, we report on the design of a setup for simultaneous culture of up to nine electrospun membranes or thin polymer films in custom-made holders under flow on an orbital shaker. The versatile design of the device allows for the use of electrospun membranes/polymer f

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Single Cell Densitometry and Weightlessness Culture of Mesenchymal Stem Cells Using Magnetic Levitaess can be accomplished via negative magnetophoresis (diamagnetophoresis), where the applied magnetic force compensates gravitational acceleration and the density of the diamagnetic object (e.g., cell) determines its levitation height. Here we describe a portable, sensitive, and cost-effective techn

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Fluorescent Nanoclusters for Imaging of Cells/Stem Cells,There is a wide interest in targeting cancer cells using fluorescent nanoclusters (NCs) capped in protein due to excellent cell viability and photostability, one-step synthesis route, large Stokes shift, good aqueous stability, and easy functionalization capability with long lifetime. Since CD44 is

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Protocol for Determining the Induction of Human Embryonic Stem Cells into Myogenic Lineage Using Elity, muscular damage, or degenerative diseases. The stem cells can prove to be excellent clinical source of myogenic progenitor cells due to their ability of self-proliferation, renewal, and differentiation into a specific phenotype. They represent an essential component of tissue engineering along

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Preparation and Characterization of Simvastatin Nanocapsules: Encapsulation of Hydrophobic Drugs inle hydrophobic drugs which are sensitive to hydrolytic conditions is of high interest in biomedical and pharmaceutical industries. The encapsulation can improve the drug solubility while decreases its side effects besides controlling its pharmacokinetic profile which results in the overall improveme

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Multifunctional Magnetic Nanoparticles-Labeled Mesenchymal Stem Cells for Hyperthermia and Bioimagiies, comparable size to biomolecules, favorable conjugations of drugs and biomolecules, ability to labeling, and capability of sensing, separation, detection, and targeted drug delivery. They could be exploited in magnetic resonance imaging as the contrast agents and also warmed as exposed to an ext