Titlebook: RNA-RNA Interactions; Methods and Protocol Frank J. Schmidt Book 2015 Springer Science+Business Media New York 2015 RNA.RNA-RNA interaction

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Modification Interference Analysis of the Ribosome,ify the nucleotides in the RNA molecule that are important for its structure and function. There are several biochemical methods such as footprinting, cross-linking, and modification interference analysis that can be used to study RNA–RNA and RNA–protein interactions. Ribosome is a classical example

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,Electrophoretic Mobility Shift Assay of RNA–RNA Complexes,NA complexes can be further used to evaluate the specificity of interactions using competitor RNAs in combination with their mutated versions or nonspecific RNAs, such as yeast tRNA. RNA is simply prepared by in vitro transcription from PCR product templates. Detailed experimental descriptions for E

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Structural Studies of a Double-Stranded RNA from Trypanosome RNA Editing by Small-Angle X-Ray Scattolution structure to the crystal structure to assess the impact of the crystal lattice on the overall conformation of the RNA. The RNA was designed to self-anneal and form a head-to-head fusion of two identical mRNA/oligo(U) tail domains (the U-helix) from a trypanosome RNA editing substrate formed

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Fusion RNAs in Crystallographic Studies of Double-Stranded RNA from Trypanosome RNA Editing,randed helix with a twofold rotation axis relating the two strands. These symmetrical RNA molecules are more likely to crystallize without end-on-end statistical packing disorder because the two halves of the molecule are identical. This approach can be used to study many fragments of double-strande

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RNA-Directed Recombination of RNA In Vitro,ques. Here, we describe the use of the . group I intron ribozyme to recombine shorter RNAs into longer ones. This ribozyme is a generalized RNA recombinase ribozyme that operates rapidly and with high efficiency.

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