Titlebook: RNA-RNA Interactions; Methods and Protocol Frank J. Schmidt Book 2015 Springer Science+Business Media New York 2015 RNA.RNA-RNA interaction

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Transactivation of Large Ribozymes,s contribute to the overall structure of the ribozyme. These elements can also be deleted, reducing catalytic activity, and their structure–function relationships determined by assaying the ability of variant elements to activate the deleted ribozyme in trans. This is illustrated for an unusual loop structure from . Ribonuclease P RNA.

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Book 2015a guidebook to scientists who are experienced with RNA research and want to brush up on a new technique. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-st

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Modification Interference Analysis of the Ribosome, of a RNA–protein complex that has been extensively studied using these methods. Here, we describe a modification interference method that was used to identify bases in 16S rRNA that are important for the translocation of the mRNA–tRNA complex by the ribosome.

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Book 2015ep, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls..Thorough and intuitive, .RNA-RNA Interactions: Methods and Protocols .guides scientists investigating biological systems and studying RNA. .

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,Identifying RNA Recombination Events and Non-covalent RNA–RNA Interactions with the Molecular Colons solid or semi-solid medium, such as a gel, which contains a system for the exponential multiplication of RNA or DNA. As an individual colony comprises many copies of a single molecule (a molecular clone), the method can be used for the detection, enumeration, and analysis of individual DNA or RNA

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,RNA–RNA SELEX,tural motifs. RNA aptamers are mainly resolved with affinity column chromatography and electrophoretic mobility shift assay (EMSA). Here, we describe the separation of the RNA aptamers binding to an RNA stem-loop target with affinity chromatography using the column attached the target RNA and nonden

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,Identification of Antisense RNA Stem-Loops That Inhibit RNA–Protein Interactions Using a Bacterial ecules that regulate specific RNA–protein interactions may be used to dissect biological processes, and to establish the validity of targeting an RNA–protein interaction. There are many examples of biological regulation by antisense RNA stem-loops that form loop-loop and loop-linear RNA–RNA interact