Titlebook: RNA-Chromatin Interactions; Methods and Protocol Ulf Andersson Vang ?rom Book 2020 Springer Science+Business Media, LLC, part of Springer N

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,Transcriptome-Wide Mapping of Protein–RNA Interactions,e regulation of these processes. The strategy called CLIP-seq (cross-linking and immunoprecipitation) has been developed to capture endogenous protein–RNA interactions with UV cross-linking followed by immunoprecipitation. Despite the wide use of conventional CLIP-seq method in RBP study, the CLIP e

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Proximity RNA-seq : A Sequencing Method to Identify Co-localization of RNA,n which RNA molecules are sequenced in their spatial, cellular context to derive RNA co-localization and transcriptome organization. Transcripts in individual subcellular particles from chemically crosslinked cells are tagged with the same, unique DNA barcode in water-in-oil emulsion droplets. First

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Characterization of R-Loop Structures Using Single-Molecule R-Loop Footprinting and Sequencing,id and a looped-out single-stranded DNA (ssDNA) strand. These structures are important for normal cellular processes and aberrant R-loop formation has been implicated in a number of pathological outcomes, including certain cancers and neurodegenerative diseases. Mapping R-loops has primarily been pe

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,Analyzing RNA–DNA Triplex Formation in Chromatin,e. Mechanisms of direct and indirect RNA-chromatin interactions have been described, including the sequence-specific formation of triple helix structures. Triplexes are formed by the sequence-specific binding of RNA to the bases located in the major groove of DNA. We recently showed that triplexes d

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,Mapping RNA–Chromatin Interactions In Vivo with RNA-DamID,in vivo and many interact with chromatin to regulate gene expression. However, the limited sensitivity of RNA-purification techniques has precluded the identification of genomic targets of cell-type specific lncRNAs. RNA-DamID is a powerful new approach to understand the mechanisms by which lncRNAs

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Book 2020tion and manipulation of gene expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubl

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Ewan K. S. McRae,David E. Davidson,Sean A. McKennases with solutions make the text suitable for advanced courses or individual study. This completely updated revised new edition contains an enlarged chapter on quantum chromodynamics and a widely updated chapter on neutrino physics..978-3-642-26951-6978-3-642-20241-4Series ISSN 1868-4513 Series E-ISSN 1868-4521