Titlebook: RNA-Chromatin Interactions; Methods and Protocol Ulf Andersson Vang ?rom Book 2020 Springer Science+Business Media, LLC, part of Springer N

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Identification of Chromatin Binding Sites for Long Noncoding RNAs by Chromatin Oligo-Affinity Preciey regulate gene expression. Chromatin oligo-affinity precipitation (ChOP) technique was the first method developed to analyze the association of LncRNAs with genomic regions in the chromatin context. The first step of the procedure is cell cross-linking, aimed at stabilizing the RNA–protein–DNA com

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Knockdown of Nuclear lncRNAs by Locked Nucleic Acid (LNA) Gapmers in Nephron Progenitor Cells,understood. To characterize the function of lncRNAs, knockdown studies are essential. However, the conventional silencing methods for mRNA, such as RNA interference (RNAi), may not be as efficient against lncRNAs, partly due to the mismatch of the localization of lncRNAs and RNAi machinery. To circu

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Simultaneous Detection of RNAs and Proteins with Subcellular Resolution,red cells and formal-fixed, paraffin-embedded (FFPE) tissue sections. On cultured cells, we detect specific transcripts using the Stellaris fluorescence in situ hybridization (FISH) probes labeled with fluorophores that target multiple regions along the desired transcripts and proteins combining the

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Proteomics-Based Systematic Identification of Nuclear Proteins Anchored to Chromatin via RNA,hromatin is likely to be covered with many RNA molecules..Here we describe a simple, reliable, and cross-link-free method for the systematic identification of chromatin-associated RBPs that exhibit RNA-dependent chromatin association.

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