Titlebook: Nanotechnology for Nucleic Acid Delivery; Methods and Protocol Manfred Ogris,Haider Sami Book 2019Latest edition Springer Science+Business

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Sequence-Defined Cationic Lipo-Oligomers Containing Unsaturated Fatty Acids for Transfection,tives under aqueous conditions. Here we describe an optimized cleavage protocol (precooling cleavage solution to 4?°C, 20?min cleavage at 22?°C), which minimizes TFA adduct formation, retains the unsaturated hydrocarbon chain character, and ensures high yields of the synthesis.

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Layer-By-Layer Film Engineering for Sequential Gene Delivery,rescence spectroscopy. Insertion of barrier layers in bioreducible LbL films is necessary to stabilize the interior layer structure and slow down the film degradation rate to achieve sequential gene delivery. Localized gene delivery from the LbL films is demonstrated using human embryonic kidney 293 (HEK 293) cells.

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1064-3745 etailed second edition volume expands upon the prior edition by addressing newly emerged technologies as well as improved existing protocols in nucleic acid delivery via nanotechnology. The book addresses topics ranging from chemical synthesis of macromolecules and bioconjugates, novel and establish

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Manfred Ogris,Haider SamiIncludes cutting-edge techniques.Provides step-by-step details essential for reproducible results.Contains key advice from the experts

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Springer Science+Business Media, LLC, part of Springer Nature 2019

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Nanotechnology for Nucleic Acid Delivery978-1-4939-9092-4Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Synthetic Oligonucleotides in SPECT/CT In Vivo Imaging: Chemical Modifications, In111 Complex Formaeprotection, isolation, and purification. We also present methods for oligonucleotides/In. complex formulation with single and double stranded oligonucleotides of RNA nature and give an example of preparation method for one supramolecular drug delivery system (DDS) consisting of radiolabeled siRNA and positively charged peptide.

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