Titlebook: NF-κB Transcription Factors; Methods and Protocol Guido Franzoso,Francesca Zazzeroni Book 2021 Springer Science+Business Media, LLC, part o

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High-Throughput Analysis of the Cell and DNA Site-Specific Binding of Native NF-κB Dimers Using Nuclcharacterize the DNA binding of transcription factors present in cell nuclear extracts. NextPBMs allow for sensitive analysis of the DNA binding of NF-κB dimers and their interactions with cell-specific cofactors.

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1064-3745 ratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and practical, .NF-κB Transcription Factors: Methods and Protocols. 978-1-0716-1671-0978-1-0716-1669-7Series ISSN 1064-3745 Series E-ISSN 1940-6029

Free-Radical

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Heather Hook,Rose W. Zhao,David Bray,Jessica L. Keenan,Trevor Siggers

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Methods to Study CARD11-BCL10-MALT1 Dependent Canonical NF-κB Activation in Jurkat T Cellsiral transduction protocol. In addition, we explain the use of a stable NF-κB-dependent EGFP reporter system that enables a reliable quantification of NF-κB transcriptional activation in the reconstituted KO Jurkat T cells.

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Analysis of Calcium Control of Canonical NF-κB Signaling in B Lymphocytescells (NFAT). NFAT is expressed ubiquitously and the mechanism by which Ca. regulates NFAT activation and signaling is well known. By contrast, a mechanistic understanding of how Ca. signals also shape the activation and specificity of NF-κB to control the expression of pro-inflammatory genes has la

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