Titlebook: NF-κB Transcription Factors; Methods and Protocol Guido Franzoso,Francesca Zazzeroni Book 2021 Springer Science+Business Media, LLC, part o

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書(shū)目名稱NF-κB Transcription Factors影響因子(影響力)




書(shū)目名稱NF-κB Transcription Factors影響因子(影響力)學(xué)科排名




書(shū)目名稱NF-κB Transcription Factors網(wǎng)絡(luò)公開(kāi)度




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書(shū)目名稱NF-κB Transcription Factors被引頻次




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Biochemical Methods to Analyze the Subcellular Localization of NF-κB Proteins Using Cell Fractionatiing their contents, thus allowing an enrichment of the protein of interest. Using different concentrations of sucrose or detergent buffer formulations in combination with centrifugations, the cell fractions are separated based on their density and size.

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Methods for Modulating the Pathway of NF-κB Using Short Hairpin RNA (ShRNA)lack of targeting drugs, or because of the risk of wider off-target effects. Here we describe the design and use of short hairpin RNA (ShRNA) and lentiviral vectors as an efficient technique for silencing NF-kappaB (NF-κB) pathway in cultured cells. This method can be used also in hard to transfect primary cell cultures.

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Measuring NF-κB Phosphorylation and Acetylationcontrol the transcriptional outcomes of this important transcription factor. These modifications work independently, sequentially or in combination to modulate the diverse biological functions of NF-κB in cancer and inflammatory response. Here, we describe some experimental methods to detect the in