Titlebook: NF-κB Transcription Factors; Methods and Protocol Guido Franzoso,Francesca Zazzeroni Book 2021 Springer Science+Business Media, LLC, part o

選民

書目名稱NF-κB Transcription Factors影響因子(影響力)




書目名稱NF-κB Transcription Factors影響因子(影響力)學科排名




書目名稱NF-κB Transcription Factors網絡公開度




書目名稱NF-κB Transcription Factors網絡公開度學科排名




書目名稱NF-κB Transcription Factors被引頻次




書目名稱NF-κB Transcription Factors被引頻次學科排名




書目名稱NF-κB Transcription Factors年度引用




書目名稱NF-κB Transcription Factors年度引用學科排名




書目名稱NF-κB Transcription Factors讀者反饋




書目名稱NF-κB Transcription Factors讀者反饋學科排名

不可侵犯

exceptional

Pruritus

BILE

步兵

Biochemical Methods to Analyze the Subcellular Localization of NF-κB Proteins Using Cell Fractionatiing their contents, thus allowing an enrichment of the protein of interest. Using different concentrations of sucrose or detergent buffer formulations in combination with centrifugations, the cell fractions are separated based on their density and size.

芭蕾舞女演員

有效

Methods for Modulating the Pathway of NF-κB Using Short Hairpin RNA (ShRNA)lack of targeting drugs, or because of the risk of wider off-target effects. Here we describe the design and use of short hairpin RNA (ShRNA) and lentiviral vectors as an efficient technique for silencing NF-kappaB (NF-κB) pathway in cultured cells. This method can be used also in hard to transfect primary cell cultures.

大暴雨

cardiovascular

Measuring NF-κB Phosphorylation and Acetylationcontrol the transcriptional outcomes of this important transcription factor. These modifications work independently, sequentially or in combination to modulate the diverse biological functions of NF-κB in cancer and inflammatory response. Here, we describe some experimental methods to detect the in