Titlebook: Metabolic Reprogramming; Methods and Protocol Salvatore Papa,Concetta Bubici Book 2023 The Editor(s) (if applicable) and The Author(s), und

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In Vivo Tissue Lipid Uptake in Antisense Oligonucleotide (ASO)-Treated Mice,al gavage. To ascertain more precisely the capacity of each tissue for fatty acid uptake, not considering the intestinal barrier, the intravenous (IV) administration of radiolabeled lipids is also described.

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Quantitation of Glutamine Synthetase 1 Activity in ,formation with FeCl.. Fixed-time assays and those based on monitoring the time-course of product formation at different reaction times are described. The protocols can be adapted to quantify glutamine synthetase activity on biological materials other than ..

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Isolation of Mitochondria from Mouse Tissues for Functional Analysis,ation from different tissues to optimize mitochondrial yield and function. This protocol offers a unified resource for preparations of isolated mitochondria from mouse liver, kidney, heart, brain, skeletal muscle, and brown and white adipose tissue suitable for functional analysis.

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Quantitation of Glutathione and Oxidized Glutathione Ratios from Biological Matrices Using LC-MS/MSs an ideal method to measure the GSH/GSSG ratio and can be applied to a variety of biological matrices and disease models. The following chapter details the design, optimization, and execution of a liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay to measure the GSH/GSSG ratio.

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Metabolite Analyses Using Nuclear Magnetic Resonance (NMR) Spectroscopy in Plasma of Patients with n addition, the detection of total proteins, lipoproteins, and signals arising from the glycosylation of circulating acute-phase proteins. Here, we describe the usage of NMR spectroscopy for profiling the plasma or serum of patients with prostate cancer.