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Titlebook: Metabolic Reprogramming; Methods and Protocol Salvatore Papa,Concetta Bubici Book 2023 The Editor(s) (if applicable) and The Author(s), und

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書目名稱Metabolic Reprogramming
副標(biāo)題Methods and Protocol
編輯Salvatore Papa,Concetta Bubici
視頻videohttp://file.papertrans.cn/632/631255/631255.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Metabolic Reprogramming; Methods and Protocol Salvatore Papa,Concetta Bubici Book 2023 The Editor(s) (if applicable) and The Author(s), und
描述.This volume provides state-of-the-art methods and protocols from researchers in the field of metabolism. Chapters guide readers through methodologies measuring different aspects of lipid metabolism, quantifying the amino acids, mitochondrial and intracellular by-products, detect enzyme and kinase activities, detecting a variety of metabolic pathways, and intracellular networks including nucleotide metabolism and the metabolic response to hypoxia and cytotoxic drugs. Written in the format of the highly successful?.Methods in Molecular Biology?.series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols...?..Authoritative and cutting-edge,?.Metabolic Reprogramming: Methods and Protocols .aims to be a?useful and practical guide to new researchers and experts looking to expand their knowledge.?.
出版日期Book 2023
關(guān)鍵詞GEO DataSets; metabolic reprogramming; Mitobiogenesis; PET/CT; rate-limiting enzymes
版次1
doihttps://doi.org/10.1007/978-1-0716-3247-5
isbn_softcover978-1-0716-3249-9
isbn_ebook978-1-0716-3247-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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Measurement of Fatty Acid Oxidation by High-Resolution Respirometry: Special Considerations for Anatissues/fibers are suspended in oxygenated respiration medium within a closed chamber and substrates or inhibitors added in a stepwise manner. The dissolved oxygen concentration decreases as aerobic metabolism in the specimen proceeds, recorded by an oxygen sensor within the chamber to give a quanti
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Quantification of Intracellular ATP Content in Ex Vivo GC B Cells,changes in the cell, leading to an increase in ATP production to sustain cell proliferation and differentiation. Current methods available to determine the amount of ATP are time-consuming, require extensive sample processing, and need a large amount of starting material. We set up an easy follow-up
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UHPLC-HRMS-Based Analysis of S-Hydroxymethyl-Glutathione, GSH, and GSSG in Human Cells,tasis. This moiety can react with cellular electrophiles such as formaldehyde yielding the compound S-hydroxymethyl-GSH (HSMGSH). HSMGSH is the substrate of the enzyme alcohol dehydrogenase 5 (ADH5) and thus a key intermediate in formaldehyde metabolism. In this work, we describe a method for the ch
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