Titlebook: Membrane Protein Structure and Function Characterization; Methods and Protocol Jean-Jacques Lacapere Book 2017 Springer Science+Business Me

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Reconstitution of Membrane Proteins into Nanodiscs for Single-Particle Electron Microscopy,ess to structural methodologies developed for soluble proteins. Here, we present the reconstitution of OprM membrane protein into nanodisc associated with a step of size-exclusion chromatography, an approach applicable to prepare IMPs for subsequent visualization by single-particle electron microscopy.

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Direct Extraction and Purification of Recombinant Membrane Proteins from , Protoplasts,ne proteins (MPs), leading to the crystallization and structure determination for a variety of them. The actual overexpression of functional MPs achieved with this system is, however, often accompanied by the formation of a variable but significant proportion of misfolded and/or aggregated proteins

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Methyl-Specific Isotope Labeling Strategies for NMR Studies of Membrane Proteins,provide high sensitivity even in very large proteins, such as membrane proteins in a membrane-mimicking environment. In this chapter, we discuss the approach for labeling methyl groups in .-based protein expression, as exemplified with the mitochondrial carrier GGC.