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Titlebook: Membrane Protein Structure and Function Characterization; Methods and Protocol Jean-Jacques Lacapere Book 2017 Springer Science+Business Me

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書目名稱Membrane Protein Structure and Function Characterization
副標(biāo)題Methods and Protocol
編輯Jean-Jacques Lacapere
視頻videohttp://file.papertrans.cn/631/630382/630382.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Membrane Protein Structure and Function Characterization; Methods and Protocol Jean-Jacques Lacapere Book 2017 Springer Science+Business Me
描述.In this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane. Since every membrane protein behaves mostly in a unique way /fashion, knowledge of guidelines and tricks may help to increase chances to express, purify and characterize a peculiar membrane protein. Production of correctly folded protein remains a challenge. Moreover, getting a functional and stable protein requires to optimize membrane mimicking environments that can be detergent or artificial membranes. In some cases, the finding of the correct ligand which will stabilize the desired conformation is needed. In other cases, stabilization can be obtained using specific antibodies. This volume also presents different techniques to analyze the functional status of membrane proteins. Written in the highly successful .Methods in Molecular Biology .series format, chapters in .Membrane Protein Structure andFunction Characterization: Methods and Protocols .provide. .different techniques to analyze the functional and structural status of membrane proteins. ?Chapters include introductions to their respective topics
出版日期Book 2017
關(guān)鍵詞membrane protein; folded protein; membrane mimicking environments; functional status; antibodies
版次1
doihttps://doi.org/10.1007/978-1-4939-7151-0
isbn_softcover978-1-4939-8399-5
isbn_ebook978-1-4939-7151-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media LLC 2017
The information of publication is updating

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Mass Spectrometry of Mitochondrial Membrane Protein Complexes, (2) provides convenient samples for bottom-up proteomics. Combining BN-PAGE and LC-MS/MS leads to the identification of the subunit composition of membrane complexes and offers the possibility of highlighting potential interacting proteins.
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1064-3745 ation advice from the experts.Includes supplementary materia.In this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane. Since every membrane protein behaves mostly in a unique way /
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Functional Studies on Membrane Proteins by Means of H/D Exchange in Infrared: Structural Changes inuch slower exchange when involved in H-bonded structures or when sterically inaccessible to the solvent. Here, we describe the protocol that was used to study the effect of phospholipids on the overall structure of the Na. NQR from ., a sodium pumping membrane protein.
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Reconstitution of Membrane Proteins in Liposomes,. This tri-partite system, which by-passes the periplams, is involved in antibiotic resistance. First, we describe a protocol to study MexB, the actual transporter, and second we propose an alternate protocol where the tripartite system is investigated as a whole.
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