Titlebook: Mammary Stem Cells; Methods and Protocol Maria dM. Vivanco Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under e

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Jakub Sumbal,Thorarinn Gudjonsson,Gunnhildur Asta Traustadottir,Zuzana Koledova

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Risa Burr,Jon F. Edd,Brian Chirn,Avanish Mishra,Daniel A. Haber,Mehmet Toner,Shyamala Maheswaran

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Computational Methods to Identify Cell-Fate Determinants, Identity Transcription Factors, and Nicher bulk RNA sequencing data for elucidating the molecular underpinnings of cell subpopulation identities, lineage specification, and the process of cell-fate decisions. Furthermore, we discuss how these computational methods provide the right framework for computational modeling of biological systems

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Modification of Single Cells Within Mouse Mammary Gland Derived Acini via Viral Transduction,thod, a few cells within the preserved organoid can be marked, changed, and tracked within an unaltered neighboring environment of non-transduced cells to better understand processes like, for instance, tumor initiation.

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Single Organoids Droplet-Based Staining Method for High-End 3D Imaging of Mammary Organoids,rvation of the 3D cellular context, but conventional confocal microscopy of organoid cultures struggles with the large organoid sample size and relatively long distance from the objective to the organoid due to the 3D extracellular matrix (ECM) that surrounds the organoid. We have overcome these iss

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interdisciplinary in nature and the contributors correspondingly include physicists, materials scientists and engineers. Likewise the book will appeal to scientists from all these areas..978-3-642-06257-5978-3-540-31631-2Series ISSN 0933-033X Series E-ISSN 2196-2812

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