Titlebook: Mammary Stem Cells; Methods and Protocol Maria dM. Vivanco Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under e

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Protocol for Studying Embryonic Mammary Gland Branching Morphogenesis Ex Vivo,toring of growth and branching morphogenesis of mammary gland ex vivo by live imaging. In addition, we introduce a novel method for culturing intact, stroma-free mammary rudiments from late gestation mouse embryos in 3D in Matrigel. This approach can be used to identify the direct stromal cues for branching morphogenesis.

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Divide and Conquer: Isolating Cell Populations to Investigate How Breast Cancer Risk Factors Alter further cell populations, such as macrophages, can be isolated for molecular analysis. These methods can be adapted to sequentially isolate other cell populations based on specific cell surface markers and are useful for small-sized breast tissue specimens.

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Patient-Derived Explant Cultures of Normal and Tumor Human Breast Tissue,potential to improve preclinical testing by contributing to provide more accurate data reflecting cell–cell and cell–matrix interactions, tumor microenvironment, drug effects or stem cell function in normal- and pathophysiology of the breast.

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Negative-Selection Enrichment of Circulating Tumor Cells from Peripheral Blood Using the Microfluidto downstream single-cell analytics including RNA-Seq, ATAC-Seq and DNA methylation, droplet digital PCR (ddPCR) for tumor specific transcripts, staining and extensive image analysis, and ex vivo culture of patient-derived CTCs.

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Lineage Tracing Methods to Study Mammary Epithelial Hierarchies In Vivo,scuss their respective advantages and inconveniences; we will then provide stepwise instructions for setting up and evaluate lineage tracing experiments, listing the most common downstream analyses and read-out assays.