Titlebook: Mammary Stem Cells; Methods and Protocol Maria dM. Vivanco Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under e

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Patient-Derived Explant Cultures of Normal and Tumor Human Breast Tissue,ypes and the use of diverse types of matrices in an attempt to mimic conditions that more closely resemble those found in the original tissue or organ. In this chapter, we describe how patient-derived breast tissue can be cultured on sponges for several days, maintaining their original architecture

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Measuring Mechanical Properties of Breast Cancer Cells with Atomic Force Microscopy,cells are tightly linked to biochemical signaling pathways related to cell structure, proliferation, differentiation, motility, and cell fate. This chapter aims to describe how to perform mechanical experiments on MCF-7 breast cancer cells using the atomic force microscope. We present a stepwise pro

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https://doi.org/10.1007/978-1-0716-2193-6ChIP-Seq; Epigenetics; Xenotransplantation; Organoid; Lentivirus

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Maria dM. VivancoIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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978-1-0716-2195-0The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines

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Book 2022Latest editionmary epithelial and stem cells, which will contribute to understand the heterogeneity of the mammary gland and of breast cancer. Chapters detail processes used to characterize stem cells, single cell RNA sequencing, computational methods, sophisticated imaging techniques, and a variety of model syst