Titlebook: Lysosomes; Methods and Protocol Karin ?llinger,Hanna Appelqvist Book 2017 Springer Science+Business Media LLC 2017 apoptosis.cell repair.de

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Delivery of Cargo to Lysosomes Using GNeosomes,alf-lives and minimized degradation. Here, we present a method for improving the selective delivery of liposomes to the lysosomes using a guanidinylated neomycin (GNeo) transporter. The method for synthesizing GNeo-lipids, incorporating them into liposomes, and the enhanced lysosomal delivery of enc

獸群

Collision

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連詞

Detection of Lysosomal Exocytosis by Surface Exposure of Lamp1 Luminal Epitopes,exocytosis in mammalian cells, which takes advantage of the presence of an abundant glycoprotein, Lamp1, on the membrane of lysosomes. Lamp1 is a transmembrane protein with a large, heavily glycosylated region that faces the lumen of lysosomes. When lysosomes fuse with the plasma membrane, epitopes

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Next-Generation Sequencing Approaches to Define the Role of the Autophagy Lysosomal Pathway in Humarallel. One key application is the understanding of genetically heterogeneous and complex diseases where 50–100 different genes may converge to control the same pathways. These disorders cannot be studied using traditional approaches, based on gene-by-gene Sanger sequencing. We have set up an NGS pr

anaerobic

Aspiration

,Methods for Determination of α-Glycosidase, β-Glycosidase, and α-Galactosidase Activities in Dried on of undigested substrates from different catabolic pathways leads to cellular dysfunction. LSDs generally presents during early childhood and have a devastating impact on the families and on public health. Over the years, approaches for treatment of some LSDs have been developed with different str

entail

Prenatal Diagnosis of Lysosomal Storage Disorders Using Chorionic Villi,ochemical/molecular study in the index case is available and a strict defined protocol, specific to each individual disorder is followed. The present chapter describes the protocols for reliable and accurate prenatal enzymatic diagnoses by fluorometric and spectrophotometric methods of lysosomal sto