Titlebook: Lysosomes; Methods and Protocol Karin ?llinger,Hanna Appelqvist Book 2017 Springer Science+Business Media LLC 2017 apoptosis.cell repair.de

rupture

書目名稱Lysosomes影響因子(影響力)




書目名稱Lysosomes影響因子(影響力)學(xué)科排名




書目名稱Lysosomes網(wǎng)絡(luò)公開度




書目名稱Lysosomes網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Lysosomes被引頻次




書目名稱Lysosomes被引頻次學(xué)科排名




書目名稱Lysosomes年度引用




書目名稱Lysosomes年度引用學(xué)科排名




書目名稱Lysosomes讀者反饋




書目名稱Lysosomes讀者反饋學(xué)科排名

消極詞匯

Detection of Lysosomal Exocytosis by Surface Exposure of Lamp1 Luminal Epitopes,d cells using highly specific monoclonal antibodies and fluorescence microscopy. The main advantage of this method is its sensitivity, and the fact that it provides spatial information on lysosomal exocytosis at the single cell level.

露天歷史劇

EXPEL

鋼盔

Analysis of ,- and ,-Glycosylation of Lysosomal Glycoproteins, specific glycosidases followed by a western blot analysis of a protein of interest provides a way to determine which types of glycans are present in the protein by comparing the gel mobility before and after treatment.

Derogate

會犯錯誤

Analysis of Lysosomal pH by Flow Cytometry Using FITC-Dextran Loaded Cells,c point, thereby the ratio can be used to determine the lysosomal pH. A standard curve is obtained by equilibrating intralysosomal pH with extracellular pH using the ionophore nigericin. The protocol also includes information regarding procedures to induce lysosomal alkalinization and lysosomal membrane permeabilization.

PALL

Quantitative Profiling of Lysosomal Lipidome by Shotgun Lipidomics,al lipidome by quantitatively detecting hundreds of lipid species of diverse physicochemical properties. We describe here a detailed protocol that couples isolation of superparamagnetic iron dextran-loaded lysosomes from cultured mammalian cell lines with quantitative mass spectrometry-based shotgun lipidomics.

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