Titlebook: Liver Proteomics; Methods and Protocol Djuro Josic,Douglas C. Hixson Book 2012 Springer Science+Business Media New York 2012 Golgi complexe

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Plasma Membrane Isolation Using Immobilized Concanavalin A Magnetic Beads,od takes advantage of the differential density of organelles. While differential centrifugation methods are sufficient to enrich for plasma membranes, the procedure is lengthy and results in low recovery of the membrane fraction. Importantly, there is significant contamination of the plasma membrane

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Producing a One-Dimensional Proteomic Map for Human Liver Cytochromes P450, polyacrylamide gel electrophoresis (SDS-PAGE) fractionation with subsequent mass spectrometric (MS) identification. The approach involves cutting an SDS-PAGE gel lane into thin slices and identifying proteins in each slice by MS with the aim of obtaining detailed information on proteins of interest

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Assessing Heterogeneity of Peroxisomes: Isolation of Two Subpopulations from Rat Liver,zation of peroxisome species from different subcellular prefractions should help to solve the question of whether peroxisomes indeed diverge into functionally specialized subgroups in one tissue. As a means to address this question, we provide a detailed separation protocol for the isolation of pero

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