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Titlebook: Liver Proteomics; Methods and Protocol Djuro Josic,Douglas C. Hixson Book 2012 Springer Science+Business Media New York 2012 Golgi complexe

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書目名稱Liver Proteomics
副標(biāo)題Methods and Protocol
編輯Djuro Josic,Douglas C. Hixson
視頻videohttp://file.papertrans.cn/588/587434/587434.mp4
概述Outlines strategies used to characterize liver proteome at the global, cellular, subcellular, post transitional, and functional level.Provides step-by-step detail essential for reproducible results.Co
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Liver Proteomics; Methods and Protocol Djuro Josic,Douglas C. Hixson Book 2012 Springer Science+Business Media New York 2012 Golgi complexe
描述.The liver is responsible for a wide range of critical functions essential to life, and is composed of several different cell types. In? .Liver Proteomics: Methods and Protocols., expert researchers in the field detail many of the methods that are used to study the live. These methods include the ?most up-to-date strategies being used to characterize the liver proteome at the global, cellular, subcellular, post translational and functional level.Written in the highly successful .Methods in Molecular Biology?. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls..?.Authoritative and practical, .Liver Proteomics: Methods and Protocols. seeks to aid scientists in the further study of this crucially important organ..
出版日期Book 2012
關(guān)鍵詞Golgi complexes; Human Liver Proteome Project; cell types; hepatocyte; liver; metabolomic profiling; post
版次1
doihttps://doi.org/10.1007/978-1-61779-959-4
isbn_softcover978-1-4939-6214-3
isbn_ebook978-1-61779-959-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2012
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A Combination of Affinity Chromatography, 2D DIGE, and Mass Spectrometry to Analyze the Phosphoproty column was an effective method for enriching phosphate-containing proteins. Further validating the method, this workflow was applied to probe changes in the activation patterns of intermediates involved in different signaling pathways, such as NDRG1 and stathmin, in liver progenitor cells (MLP-29) upon proteasome inhibition.
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Producing a One-Dimensional Proteomic Map for Human Liver Cytochromes P450,each slice. Our protocol proved to be efficient enough to obtain a comprehensive profile of drug-metabolizing enzymes in the human liver. In addition to human tissues, the approach described should be applicable to the characterization of membrane proteins in other eukaryotic species.
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