Titlebook: Interleukin Protocols; Luke A. J. O’Neill,Andrew Bowie Book 2001 Humana Press 2001

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Assaying Interleukins Intracellularly by FACScated instruments. These instruments are now used widely in all branches of biologic science. Originally, the preserve of the research laboratory, flow cytometers are now playing an increasingly important role in both clinical and industrial laboratories.

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Basic RT-PCR for Measurement of Cytokine Expressionxpression of mRNA for a particular cytokine does not imply corre-sponding expression of the protein, both techniques in combination provide important, specific information in a short time. Since the late 1980s, the poly-merase chain reaction (PCR), a powerful and exquisitely sensitive technique, has

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Quantitative PCR for Measurement of Cytokine Expressionh aberrant production of one or more cytokines. It is therefore of considerable interest to characterize cytokine “profiles” associated with disease processes. However, the minute amounts of cytokine protein often produced in autocrine or paracrine microenvironments may not be easily detectable, esp

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Isolation of Total and Bioactive Interleukins by Immunoaffinity-Receptor Affinity Chromatographyprocedure isolates the total amount of a specific cytokine, and the latter procedure isolates the bioactive fraction of the immunoaffinity-purified material. Recombinant receptors are becoming available for a number of different biologic materials and are available in forms suitable for affinity chr

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Detection of Cytokines by Immunohistochemistry of nonimmune cell origin such as epithelial and muscle cells are also capable of producing cytokines, as well as many tumor cells (., .). We have recently demonstrated that sperm cells are capable of producing interleukin-1 (IL-1) (.). The autocrine and paracrine effects excerted by cytokines may t

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Assaying Interleukin-8h the capacity to act as leukocyte chemoattractants (.). Chemokines are crucial mediators of inflammation as they are able to enhance migration selectively of specific types of leukocytes from blood vessels into damaged and/or infected tissue, where the leukocytes will effect elimination of the infe