Titlebook: Immune Mediators in Cancer; Methods and Protocol Ivana Vancurova,Yan Zhu Book 2020 Springer Science+Business Media, LLC, part of Springer N

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Methods for Studying TNFα-Induced Autophagy autophagy process maintains protein homeostasis by recycling damaged organelles and degrading many long-lived proteins in conjunction with the ubiquitin-proteasome system. Cytokines are low-molecular-weight secreted proteins that regulate a broad range of biological activities. For instance, pro-in

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Isolation of Antibody Binders to MISIIR from a Phage Display Library by Sorting antibody repertoires, such as immunized or na?ve phage display libraries, has been a challenging task, which is an outstanding issue when soluble portion of the target(s) is not available, and/or a na?ve phage display library is used. Common cell-based panning methods often encounter numerous diffi

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Probing Metabolic Changes in IFNγ-Treated Ovarian Cancer Cells and regulates different aspects of cell physiology, including metabolism. Cancer cells undergo a complex rearrangement of metabolic pathways that allows them to satisfy the needs of increased proliferation, and many cancer cells redirect glucose metabolism from oxidative phosphorylation to aerobic

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Luminex xMAP Assay to Quantify Cytokines in Cancer Patient Serumsing Luminex. 200? analyzer with xPonent. acquisition software to quantify cytokines, chemokines, and growth factors secreted to blood serum and plasma of cancer patients. We also discuss how sample preparation, instrument settings, and standard curve fitting algorithms can influence validity of obt

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