Titlebook: Immune Mediators in Cancer; Methods and Protocol Ivana Vancurova,Yan Zhu Book 2020 Springer Science+Business Media, LLC, part of Springer N

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Analysis of Circulating HMGB1 in Human Serumas well as passively released from damaged cells following pathological injuries. Studies indicate that HMGB1 functions as a mediator of infection- and injury-elicited inflammatory diseases. Although intracellular HMGB1 functions as a regulator of tumorigenesis, epigenetic anticancer agents or thera

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Application of Immunohistochemistry in Basic and Clinical Studiesg antigens (proteins) by antibodies in cells or tissue sections. This chapter introduces the procedure and application of immunohistochemistry. Although immunohistochemistry has a vast application in basic and clinical studies, this chapter focuses on its application in biomarker study, particularly

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Luminex xMAP Assay to Quantify Cytokines in Cancer Patient Serum factors contribute to regulation of tumor growth, immune cell recruitment, angiogenesis, and metastasis. In body fluids, levels of inflammatory mediators reflect the patient immune response to the disease and may predict the effects of targeted therapies. Significant improvements in cytokine detect

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Detection of Cytokine Receptors Using Tyramide Signal Amplification for Immunofluorescenceules in situ..Here we describe immunofluorescent detection of a low-abundance cytokine receptor, interleukin-17 receptor B (IL17RB) in U2OS cells, using tyramide signal amplification. In addition, we present a tyramide signal amplification compatible double-color immunostaining protocol using primar

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