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Titlebook: Hydrocarbon and Lipid Microbiology Protocols; Ultrastructure and I Terry J. McGenity,Kenneth N. Timmis,Balbina Nogale Book 2016 Springer-Ve

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發(fā)表于 2025-3-21 17:04:20 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Hydrocarbon and Lipid Microbiology Protocols
副標題Ultrastructure and I
編輯Terry J. McGenity,Kenneth N. Timmis,Balbina Nogale
視頻videohttp://file.papertrans.cn/431/430331/430331.mp4
概述Offers readily reproducible, step-by-step laboratory methods.Provides helpful tips and tricks to complement the protocols.Includes troubleshooting advice.Features practical laboratory guidelines to pr
叢書名稱Springer Protocols Handbooks
圖書封面Titlebook: Hydrocarbon and Lipid Microbiology Protocols; Ultrastructure and I Terry J. McGenity,Kenneth N. Timmis,Balbina Nogale Book 2016 Springer-Ve
描述This Volume presents key microscopy and imaging methods for revealing the structure and ultrastructure of environmental and experimental samples, of microbial communities and cultures, and of individual cells. Method adaptations that specifically address problems concerning the hydrophobic components of samples are highlighted and discussed. The methods described range from electron microscopy and light and fluorescence microscopy, to confocal laser-scanning microscopy, and include experimental set-ups for the analysis of interfacial processes like microbial growth and activities at hydrocarbon:water interfaces, biofilms and microbe:mineral interfaces. Three forms of fluorescence in situ hybridization - CARD-FISH, MAR-FISH and Two-pass TSA-FISH - are described for the ecophysiological analysis of functionally active microbes in samples. The methods presented will enable readers to obtain an ultrastructural picture of, and identify the key functional microbes in, samples under investigation. This in turn will constitute a key framework for the interpretation of information from other experimental approaches, such as physicochemical analyses and genomic investigations..Hydrocarbon an
出版日期Book 2016
關鍵詞Confocal microscopy; Electron microscopy; Fluorescence imaging; Fluorescence in situ hybridization; Micr
版次1
doihttps://doi.org/10.1007/978-3-662-49134-8
isbn_softcover978-3-662-56983-2
isbn_ebook978-3-662-49134-8Series ISSN 1949-2448 Series E-ISSN 1949-2456
issn_series 1949-2448
copyrightSpringer-Verlag Berlin Heidelberg 2016
The information of publication is updating

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978-3-662-56983-2Springer-Verlag Berlin Heidelberg 2016
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發(fā)表于 2025-3-22 09:03:05 | 只看該作者
Hydrocarbon and Lipid Microbiology Protocols978-3-662-49134-8Series ISSN 1949-2448 Series E-ISSN 1949-2456
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Ultrastructure and Imaging,ific labeling approaches, however label-free imaging is a promising developing field. Light and electron microscopy, particularly when well integrated, have excellent potential to allow mechanistic insight into biological processes in hydrocarbon and lipid research.
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發(fā)表于 2025-3-22 21:51:59 | 只看該作者
Fluorescence Microscopy for Microbiology,d samples. The preparation of high-quality samples for microscopic observation is the starting point for obtaining good resolution and optimum imaging results. This chapter attempts to provide basic methods for the application of conventional fluorescence and immunofluorescence microscopy for microbiology.
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發(fā)表于 2025-3-23 04:40:24 | 只看該作者
Identification of Microorganisms in Hydrocarbon-Contaminated Aquifer Samples by Fluorescence In Sitnt particles, by concentrating microbial cells on membrane filters, and by using signal amplification in combination with FISH (CARD-FISH), we were able to quantify various microbial populations in this intriguing ecosystem.
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Electron Microscopy Protocols for the Study of Hydrocarbon-Producing and Hydrocarbon-Decomposing Mihe integrity of biological membranes. Here we review classical and novel advanced electron microscopy approaches, including correlative light and electron microscopy that in combination with genetics and biochemical experimentation can be utilized to study such hydrocarbon–cell interactions.
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