Titlebook: Hydrocarbon and Lipid Microbiology Protocols; Ultrastructure and I Terry J. McGenity,Kenneth N. Timmis,Balbina Nogale Book 2016 Springer-Ve

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978-3-662-56983-2Springer-Verlag Berlin Heidelberg 2016

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Hydrocarbon and Lipid Microbiology Protocols978-3-662-49134-8Series ISSN 1949-2448 Series E-ISSN 1949-2456

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Ultrastructure and Imaging,ific labeling approaches, however label-free imaging is a promising developing field. Light and electron microscopy, particularly when well integrated, have excellent potential to allow mechanistic insight into biological processes in hydrocarbon and lipid research.

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Fluorescence Microscopy for Microbiology,d samples. The preparation of high-quality samples for microscopic observation is the starting point for obtaining good resolution and optimum imaging results. This chapter attempts to provide basic methods for the application of conventional fluorescence and immunofluorescence microscopy for microbiology.

牽連

Identification of Microorganisms in Hydrocarbon-Contaminated Aquifer Samples by Fluorescence In Sitnt particles, by concentrating microbial cells on membrane filters, and by using signal amplification in combination with FISH (CARD-FISH), we were able to quantify various microbial populations in this intriguing ecosystem.

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Electron Microscopy Protocols for the Study of Hydrocarbon-Producing and Hydrocarbon-Decomposing Mihe integrity of biological membranes. Here we review classical and novel advanced electron microscopy approaches, including correlative light and electron microscopy that in combination with genetics and biochemical experimentation can be utilized to study such hydrocarbon–cell interactions.