Titlebook: Human Fungal Pathogen Identification; Methods and Protocol Thomas Lion Book 2017 Springer Science+Business Media New York 2017 Invasive fun

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Current Challenges in the Diagnosis of Fungal Infections to distinguish from invasive disease, blood cultures are commonly negative, and patients are often unable to undergo invasive diagnostic procedures. Culture and microscopic examination remain the “gold standard” but are insensitive. Antigen assays such as the galactomannan and glucan detection syst

Processes

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Commercial Molecular Tests for Fungal Diagnosis from a Practical Point of Viewd development of new methods, in particular polymerase chain reaction. High expectations have been placed on molecular diagnostics, and the number of laboratories now using the relevant technology is rapidly increasing—resulting in an obvious need for standardization and definition of laboratory org

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Fungal-Grade Reagents and Materials for Molecular Analysistially multiple input of contaminating DNA from exogenous sources. Besides airborne, handling and cross-contamination, materials and reagents used in the molecular laboratory can contain microbial DNA which is a long underestimated potential source of false positive results. In this contribution dec

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Host-Derived Biomarkers for Risk Assessment of Invasive Fungal Diseases into clinical processes to predict the risk and progression of fungal disease is a promising approach in immunocompromised patients. Recent insights into human antifungal immunity have highlighted the remarkable influence of host genetics in modulating susceptibility to infection. In this chapter,

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Histopathologymost frequently seen on skin or mucosal surfaces or as mycotic thrombi or emboli that can occlude both arteries and veins in surgical specimen from immunocompromised patients or tissues obtained from autopsies. Microbial culture continues to be the central method for diagnosing fungal infection but

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Culture-Based Techniques slow, may yield the specific etiological agent, and may allow susceptibility testing to be performed. Proper collection and transportation of the specimen is essential. Particularly, sterile materials are important for diagnosis of invasive fungal infections..Therefore, culture and direct microscop