Titlebook: Human Fungal Pathogen Identification; Methods and Protocol Thomas Lion Book 2017 Springer Science+Business Media New York 2017 Invasive fun

古生物學(xué)

書目名稱Human Fungal Pathogen Identification影響因子(影響力)




書目名稱Human Fungal Pathogen Identification影響因子(影響力)學(xué)科排名




書目名稱Human Fungal Pathogen Identification網(wǎng)絡(luò)公開度




書目名稱Human Fungal Pathogen Identification網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Human Fungal Pathogen Identification被引頻次




書目名稱Human Fungal Pathogen Identification被引頻次學(xué)科排名




書目名稱Human Fungal Pathogen Identification年度引用




書目名稱Human Fungal Pathogen Identification年度引用學(xué)科排名




書目名稱Human Fungal Pathogen Identification讀者反饋




書目名稱Human Fungal Pathogen Identification讀者反饋學(xué)科排名

協(xié)定

Histopathologysion. These stains employ oxidizing reagents to create aldehyde binding sites on polysaccharides (1,2-glycol groups) of fungal cell walls for either Schiff’s reagent or Silver ions. Gomori methenamine silver (GMS) and Periodic acid-Schiff (PAS) or their modifications are the most commonly used for tissue sections and in cytology specimens.

ANTI

征兵

被詛咒的人

Current Challenges in the Diagnosis of Fungal InfectionsCulture and microscopic examination remain the “gold standard” but are insensitive. Antigen assays such as the galactomannan and glucan detection systems are frequently used, yet these tests vary in sensitivity and specificity, depending on the patient population involved. Molecular-based assays are not yet clinically validated.

阻撓

失望未來

Isolation of Nucleic Acids for Fungal Diagnosis PCR utility will be limited by the quality of extracted nucleic acid. This chapter highlights benefits and limitations affecting the nucleic acid extraction process, before focusing on recent recommendations that through multicenter evaluation have provided optimal and standardized methodology.

Phagocytes

Prerequisites for Control of Contamination in Fungal Diagnosisgal assays. In this chapter, common origins of fungal contaminants that compromise molecular fungal testing are described, and measures for preventing contamination are proposed. Detailed guidelines for sample handling, reagent selection, contamination screening, and decontamination procedures are provided.

訓(xùn)誡

Fungal-Grade Reagents and Materials for Molecular Analysisontamination procedures of materials and reagents and the selection of certified microbial DNA-free components for sample collection, DNA extraction, and PCR amplification are discussed with respect to the aim of building up a reliable molecular system for the diagnosis of fungal organisms at the limit of detection.

手勢

Assessment of Immune Responses to Fungal Infections: Identification and Characterization of Immune Cphenotypic and functional aspects of immune cells in infected tissues is key for understanding the mechanisms of antifungal defense. In this chapter, we describe a method based on flow cytometry to assess innate and adaptive immune cells isolated from an . context in a qualitative and quantitative manner.