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Titlebook: High-Throughput Screening Assays in Toxicology; Hao Zhu,Menghang Xia Book 2022Latest edition This is a U.S. government work and not under

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樓主: Exaltation
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發(fā)表于 2025-3-27 00:19:54 | 只看該作者
32#
發(fā)表于 2025-3-27 03:27:32 | 只看該作者
GFP-LC3 High-Content Assay for Screening Autophagy Modulators also?be used to identify autophagy modulators by screening?a large number of compounds. This chapter describes a cell-based high content green fluorescent protein (GFP)-LC3 assay using mouse embryonic fibroblasts (MEF) stably expressing GFP-LC3.
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發(fā)表于 2025-3-27 08:55:34 | 只看該作者
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發(fā)表于 2025-3-27 12:03:05 | 只看該作者
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發(fā)表于 2025-3-28 01:47:17 | 只看該作者
Cell-Based Imaging Assay for Detection of Phospholipidosiscreening (HTS) platform. This assay has been optimized and validated in HepG2 and HepRG cells, and miniaturized into a 1536-well plate, thus can be used for high-throughput screening (HTS) to identify chemical compounds that induce phospholipidosis.
38#
發(fā)表于 2025-3-28 06:06:25 | 只看該作者
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發(fā)表于 2025-3-28 09:26:32 | 只看該作者
Cell-Based Assays to Identify ERR and ERR/PGC Modulatorsgonists require examination of this nuclear receptor alone and together with PGC-1α. In this book chapter, we describe the step-by-step protocol of a multiplex luciferase assay designed to identify ERRα agonists, antagonists, and toxicity in one quantitative high-throughput screening assay using two different stable cell lines.
40#
發(fā)表于 2025-3-28 12:50:55 | 只看該作者
Cell-Based hERG Channel Inhibition Assay in High-Throughput Formattivity. This chapter describes a cell-based hERG channel inhibition assay that has been optimized and performed in a 1536-well plate format. The homogeneous and robust assay can be used to identify compounds that inhibit hERG channel activity.
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