Titlebook: Heterologous Protein Production in CHO Cells; Methods and Protocol Paula Meleady Book 2017 Springer Science+Business Media LLC 2017 CRISPR.

不可磨滅

Keratin

TOXIN

迫擊炮

Cloning of Single-Chain Antibody Variants by Overlap-Extension PCR for Evaluation of Antibody Expretions and assembled by a single overlap-extension PCR reaction. The amplified product is then cloned into a mammalian expression vector suitable for high-titer transient gene expression. This workflow can be applied to any antibody sequence by adapting the specific primer sequences to the antibody of choice.

薄荷醇

Improved CHO Cell Line Stability and Recombinant Protein Expression During Long-Term Culture,e stability of the recombinant CHO cells over long-term culture. These procedures include the following; western blotting, ELISA to evaluate protein production, real-time PCR to analyze plasmid and mRNA copy numbers, and fluorescent in situ hybridization (FISH) to assess the location of the inserted plasmid on host cell chromosomes.

BYRE

6Applepolish

Large-Scale Transient Transfection of Chinese Hamster Ovary Cells in Suspension,incubation at 31 °C with agitation by orbital shaking. We also describe an alternative method in which 90% of the pDNA is replaced by nonspecific (filler) DNA, and the production phase is performed at 31 °C in the presence of 0.25% N, N-dimethylacetamide (DMA).

travail

歌劇等

Selection of High-Producing Clones Using FACS for CHO Cell Line Development,powerful, high-throughput technique that facilitates multiparametric characterization and isolation of individual cell clones from heterogeneous populations. Here, we describe a FACS-based method for section of high-producing CHO cell clones.

inquisitive

Characterization of Host Cell Proteins (HCPs) in CHO Cell Bioprocesses, while estimation of HCP clearance/presence can be achieved by comparing 2D-PAGE images of samples and by undertaking western blotting of 2D-PAGE analyzed samples. Here, we describe the analyses of HCP content using these methodologies.