Titlebook: Heterologous Protein Production in CHO Cells; Methods and Protocol Paula Meleady Book 2017 Springer Science+Business Media LLC 2017 CRISPR.

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,Strategies and Considerations for Improving Expression of “Difficult to Express” Proteins in CHO Cecould result in low protein expression, while the inherent properties of the molecule itself may limit its production via mechanisms such as cytotoxicity or inherent instability. Strategies to identify the rate-limiting step and subsequently improve expression and production are discussed here.

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Application of CRISPR/Cas9 Genome Editing to Improve Recombinant Protein Production in CHO Cells,PR-associated protein 9 (Cas9) to knockout engineering target genes in CHO cells. As an example, we refer to the glutamine synthetase (GS)-encoding gene as the knockout target gene, a knockout that increases the selection efficiency of the GS-mediated gene amplification system.

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A Bioinformatics Pipeline for the Identification of CHO Cell Differential Gene Expression from RNA-tional pipeline for the analysis of CHO cell RNA-Seq data from the Illumina platform to identify differentially expressed genes. The example data and bioinformatics workflow required to run this analysis are freely available at ..

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Book 2017s for the characterization of CHO cells using ‘omic approaches. This book also explores methods that discuss the genome editing tool, CRISPR/Cas9, and the characterization of recombinant protein products, such as glycosylation and host cell protein analysis. Written in the highly successful .Methods

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