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Titlebook: Heterologous Gene Expression in E.coli; Methods and Protocol Thomas C. Evans, Jr.,Ming-Qun Xu Book 2011 Springer Science+Business Media, LL

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書(shū)目名稱Heterologous Gene Expression in E.coli
副標(biāo)題Methods and Protocol
編輯Thomas C. Evans, Jr.,Ming-Qun Xu
視頻videohttp://file.papertrans.cn/427/426033/426033.mp4
概述Helps to guide scientists toward an ideal expression host.Provides easy to follow protocols, ready to be implemented in the lab.Includes tips from the experienced, expert contributors on troubleshooti
叢書(shū)名稱Methods in Molecular Biology
圖書(shū)封面Titlebook: Heterologous Gene Expression in E.coli; Methods and Protocol Thomas C. Evans, Jr.,Ming-Qun Xu Book 2011 Springer Science+Business Media, LL
描述Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry.Despite the advanced state of protein expression technology improvements are still needed.For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host.The ideal host would have the ability to express any protein, with relevant post-translational modifications, and be as easy to work with as E. coli.In Heterologous Gene Expression in E. coli: Methods and Protocols, expert scientists intimately familiar with the relevant techniques offer chapters that greatly expand the utility of this expression host.The contributions in this detailed volume describe methods, for example, to successfully express proteins in E. coli that would otherwise form aggregates in this host, to add post-translational modifications, to incorporate non-standard amino acid residues or moieties into E. coli expressed proteins, to identify binding partners, and to express membrane proteins.Written in the highly successful Methods in Molecular Biology? format, chapters include intro
出版日期Book 2011
關(guān)鍵詞Amino acid residues or moieties; Binding partners; Escherichia coli; Expression hosts; Membrane proteins
版次1
doihttps://doi.org/10.1007/978-1-61737-967-3
isbn_softcover978-1-4939-5725-5
isbn_ebook978-1-61737-967-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC 2011
The information of publication is updating

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