Titlebook: Hemorrhagic Fever Viruses; Methods and Protocol Maria S. Salvato Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 20

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Hemorrhagic Fever Virus Budding Studiespectively, gives rise to the production and release of virus-like particles (VLPs) that are morphologically identical to infectious virions. We can detect and quantify VLP production and egress in mammalian cells by transient transfection, SDS-PAGE, Western blotting, and live cell imaging techniques

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Roles of Arenavirus Z Protein in Mediating Virion Budding, Viral Transcription-Inhibition and Interfor virus budding from the membrane of the infected cells through self-oligomerization, a process that can be aided by the viral nucleoprotein (NP) to form the viral matrix of progeny virus particles. Z has also been shown to be essential for mediating viral transcriptional repression activity by loc

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Studies of Lassa Virus Cell Entrycond, intracellular receptor, the late endosomal/lysosomal resident protein LAMP1. Here, we describe a series of experimental approaches to investigate LASV cell entry and to test candidate inhibitors for their action at this early and decisive step of infection.

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Assays to Assess Arenaviral Glycoprotein Functiontion subunit GP1 and the transmembrane subunit GP2, which helps promote the membrane fusion reaction to allow virus entry into the cell. This article describes assays to assess PICV GPC expression, proteolytic processing, fusion function, and GPC-mediated virus-like particle (VLP) entry into cells under tissue-culture conditions.