Titlebook: Hematopoietic Stem Cell Protocols; Kevin D. Bunting Book 2008Latest edition Humana Press 2008 BAC.Flow cytometry.HSC.Lentiviral transducti

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Hematopoietic Development of Human Embryonic Stem Cells in Culturesms that mediate cell-fate decisions toward distinct developmental lineages. hESC-derived cells may also be suitable for novel cellular therapies. Significant progress in hematopoietic development of hESCs has demonstrated production of many types of blood cells from hESCs including myeloid, erythro

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In Vitro Human T Cell Development Directed by Notch–Ligand Interactionsmple in vitro protocol for generating large numbers of human T-lineage cells from umbilical cord blood (CB)- derived hematopoietic stem cells (HSCs) using a bone marrow stromal cell line. This protocol is broken into three major steps: (1) the maintenance of a working stock of OP9 bone marrow stroma

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In Vitro Assays for Cobblestone Area-Forming Cells, LTC-IC, and CFU-Cuency of progenitors (colony-forming unit in culture; CFU-C), stem cells (long-term culture-initiating cell; LTC-IC), or both (cobblestone area-forming cell assay; CAFC). These assays measure the potential of a test cell population retrospectively, i.e., at the time its activity is evident when the

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Hematopoietic Stem Cell Transplant in Mice by Intra-Femoral Injectionliver. The frequency of cells homed in bone marrow by such method is extremely low. This circumstance adds difficulty to the research of hematopoietic stem cell (HSC), a rare population to begin with. By introducing HSC directly into bone marrow cavity, the peripheral loss of HSC can be minimized. T

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Isolation and Functional Characterization of Side Population Stem Cells42 and can be used as the basis by which to isolate these cells using flow cytometry. In the bone marrow (BM), the SP defines a cell subset with a highly homogeneous content of hematopoietic stem cells (HSCs). In this chapter, we describe a protocol to reproducibly isolate murine BM SP cells, as wel

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Immunodeficient Mouse Models to Study Human Stem Cell-Mediated Tissue Repairmutations, or that had been eradicated to treat a disease such as leukemia. However, in recent years, much attention has been paid to the new concept of “stem cell plasticity,” and the hope that stem cells could be used to repair damaged tissues generated immense excitement. The field is now in a mo

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ytical tools and FEM-codes. Several tools are presented and discussed in some detail and recommendations are given where possible. Thus this chapter is valuable for all who really want to make use of the Puck model in their design work.