Titlebook: Hematopoietic Stem Cell Protocols; Kevin D. Bunting Book 2008Latest edition Humana Press 2008 BAC.Flow cytometry.HSC.Lentiviral transducti

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Sigrid Eckardt,K. John McLaughlinntation mining. Bagging and XGBoost classifiers give the best results. Fourthly, we assess the possibility of using several machine translation systems (Google Translate, Yandex.Translate and Promt) for automatic creating of argument-annotated corpora. Google Translate appears to be the best system to reach this goal.

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In Vitro Human T Cell Development Directed by Notch–Ligand Interactions maintenance/expansion of OP9-DL1 cocultures over time (...). The use of this system opens avenues for basic research as it equips us with a simple in vitro method for studying human T cell development.

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Transplantation of Chimeric Fetal Liver to Study Hematopoiesistem and progenitor cells. This chapter describes principles and methods for the generation of mouse chimeras, for identification and quantitative analysis of cell contribution in chimeras, and for chimeric fetal liver transplantation into adult recipients and analysis of mutant cells in the adult.

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The Expanding Tool Kit for Hematopoietic Stem Cell Researchen made in their isolation from mouse bone marrow to high levels of purity based on a combination of cell-surface phenotype and functional characteristics. In addition, in vitro assays have been established that provide important tools for study of hematopoietic differentiation from HSC and for diff

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Isolation of Quiescent Murine Hematopoietic Stem Cells by Homing Propertiesmitive HSCs from bone marrow. Several methods have been available including the commonly used technique of isolating HSCs based on a specific cell-surface phenotype. As surface marker expression is dynamic and may fluctuate depending on the proliferative or activation state of the cell, our laborato

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Mobilization of Hematopoietic Stem and Progenitor Cells in Micedels suggest that changes in the interaction between the HSPC and the hematopoietic microenvironmental ‘niche’ (cellular and extracellular components) are critical to the process. The increasing availability of recombinant proteins (growth factors, cytokines, chemokines), antibodies, drugs (agonists

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Transmigration of Human CD34+ Cellsof HSC to the bone marrow niche is believed to be a crucial step for engraftment. However, the molecular mechanisms that regulate HSC homing are not understood well. Migration of HSC in response to cytokines and chemokines is believed to be one of the critical steps of HSC homing and mobilization. E

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Analysis of Apoptosis in Hematopoietic Stem Cells by Flow Cytometry manipulation, or genetic mutation. Two different methods to assess the frequency of apoptosis in the HSC compartment are presented. The first method utilizes an intracellular TUNEL assay that detects DNA strand breaks, a late apoptotic event. The second method relies on an extracellular stain with