Titlebook: Germ Cell Protocols; Volume 2: Molecular Heide Schatten Book 2004 Humana Press 2004

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Differentiating Embryonic Stem Cells into Embryoid Bodies,lular basis for embryonic defects or the interpretation of knockout phenotypes, especially those complicated by early embryonic lethality. In addition, great efforts are made to define protocols to preferentially direct ES cell differentiation as EBs toward one single cell fate, therefore providing

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Pronuclear Microinjection of Mouse Zygotes,genome with the objective of creating transgenic mice. In contrast to chemical mutagenesis, where phenotypic changes are a prerequisite to identify the induced mutation (forward genetics), transgenic technology is based on the reintroduction of a previously isolated and in vitro recombined DNA seque

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Cytoplasmic Transfer in Mammalian Eggs and Embryos, understanding of basic developmental biology, but also has critical implications in animal husbandry and biotechnology (.), as well as in assisted reproductive medicine (.). Experimental manipulation of the cytoplasm of oocytes and early embryos has been a significant component of this research. Al

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Book 2004xplored in sea urchin and asc- ian eggs, now benefits cloning efficiency in farm and domestic animals. Gene manipulations and molecular methods have added to the possibilities of p- ducing live offspring with enormous biomedical, ecological, and economic implications. All sexually reproducing organi

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1064-3745 hase of new discoveries with the introd- tion of transgenic technologies and nuclear cloning. Basic knowledge and te- niques developed for lower vertebrate and invertebrate systems have facilitated the study of higher vertebrates, including humans. Many experiments that have first been performed on

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Wirtschaftspolitische Schlussfolgerungen,ns makes it possible to manipulate germline cells in vitro (e.g., DNA transfer or cryopreservation), and the manipulated germline cells can allow viable offspring by germline chimeric chickens. It is expected that endangered avian species can also proliferate via interspecific germline chimeric chickens.

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